IL-17和HSP90在不同牛肝中的定位和定量研究

doi:10.11843/j.issn.0366-6964.2019.09.018

Abstract

Abstract: The aim of this study was to study the distribution and quantification of IL-17 and HSP90 in liver tissues of different cattle, and to compare the positive reaction and differential expression of IL-17 and HSP90 in the liver tissues of Yak, Cattle-yak and Yellow cattle. Liver samples of healthy adult Yak, Cattle-yak and Yellow cattle (n=5) were selected, respectively. HE, immunohistochemical, and Quantitative Real-time PCR methods were used to observe the histological features, protein positive localization, and gene quantification of liver, respectively. Results showed that:hepatic cells were arranged in a cord-like manner, and a small number of Lymphocytes and Kupffer's cells were found in the blood sinuses between the hepatic cords (HE staining). Both IL-17 and HSP90 were expressed in the cytoplasm of liver cells of Yak, Cattle-yak and Yellow cattle (Immunohistochemical results). The results of Immunohistochemistry Semi-quantitative, and Quantitative Real-time PCR were consistent. The highest expression was found in the liver of Cattle-yak, followed by Yak, and the lowest in Yellow cattle (P < 0.01). Differential expression of IL-17 and HSP90 genes in liver of Yak, Cattle-yak and Yellow cattle shows species specificity. Semi-quantitative positive results of IL-17 and HSP90 protein showed that the liver of Yak and Cattle-yak had better adaptability to temperature differences than the liver of Yellow cattle.

CLC Number:

S852.1

ZHAO Kai, CUI Yan, LIU Penggang, HE Junfeng, SONG Liangli, LIAO Bo, YANG Xiaoqing. Localization and Expression of IL-17 and HSP90 in Different Bovine Livers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1897-1903.

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Localization and Expression of IL-17 and HSP90 in Different Bovine Livers

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